Dissolved oxygen saturation is crucial for gas bladder inflation in turquoise killifish (Nothobranchius furzeri)

Abstract Providing optimal conditions for early-life gas bladder inflation of captive fish is one the biggest challenges in culture. It also applies to laboratory fishes. Turquoise killifish ( Nothobranchius furzeri Jubb, 1971) a popular research model biogerontology due its short lifespan. Annual culture frequently suffer from an inability inflate their which may stem suboptimal environmental captivity. Here, we investigate (1) effect dissolved oxygen (DO) saturation and (2) access water surface on hatching success turquoise killifish. We further histologically examine development primordial form full inflation. In accordance with physoclistous nature killifish, not necessary found that was highest treatment constant or decreasing DO saturation. contrast, proportion larvae inflated bladders oversaturated (130%) induced by addition tablet. Larvae within 2 48 h post-hatching. These findings represent major step toward solution persistent problem this increasingly important organism.